Simple Calculation Programs for Biology Methods in Molecular Biology

• GMAP: A program for mapping potential restriction sites

• • RE sites in ambiguous and non-ambiguous DNA sequence
  • Minimum number of silent mutations required for introducing a RE sites
  • Set theory for searching RE sites
  • Raghava and Sahni (1994) Biotechniques 16:1116

• DNASIZE: Improved estimation of DNA size from Gel Electrophoresis

• • Graphical method to improved prediction
  • Raghava (1994) Biotechniques 17:100

• DNAOPT: Optimization of gel conditions of gel electrophoresis and SDS-PAGE

• • Optimization of gel conditions
  • Sufficient distance between two fragments
  • Small fragment in range
  • Raghava (1995) Biotechniques 18:274