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Table of contents
Mammalian Prolactin – An Ancient But Still A Mysterious Hormone
Prolactin inhibits LHRH action during lactational ammenorrhoea
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REDUCTIONIST VIEW OF HORMONES
CONCERN
PURIFICATION PROTOCOLS
CHARACTERIZATION OF HORMONES
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MALDI-TOF Spectrum of Monomeric PRL
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Structure of the oligosaccharide in Bu-LH-1 & Bu-LH-2
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Microheterogeneity in Protein Hormone-Structural Characteristics and Functional Significance
Biological Potency (ED50) of Different Isoforms of buPRL in The Nb2 Cell Line Growth Promotion Assay
Ammonia Liberation from PRL at Alkaline pH
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Optimization of Sandwich ELISA.
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SIGNIFICANCE OF UREASE
UREASE MONITORING SYSTEMS
Synthesis of Bispyrazolone
Bispyrazolone Method and Indophenol Method
TWO HYPOTHESES
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Induction of Recombinant BuPRL
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Fig. SWISSMODEL analysis. A. 3D structure of buPRL (http://swissmodel.expasy.org). B. Angiostatic peptide in buPRL built with hPRL. * Keeler, C., Dannies, P.S., Hodsdon, M.E., The Tertiary Structure and Backbone Dynamics of Human Prolactin. J. Mol. Biol., 328: 1105-1121, 2003. (http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1n9d)
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Anti-angiogenesis by Synthesized Peptide
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Colleagues and Collaborators