H C Saraswat
Articles written in Proceedings – Section A
Volume 30 Issue 6 December 1949 pp 329-332
The equation used by Dey for the calculation of the formula of the complex compounds from solubility data is shown to be applicable in cases where either the complex formation is very weak or in cases where
Volume 36 Issue 5 November 1952 pp 343-354
1. A new technique has been described for studying the mechanism of action of pH on spreading of invertase. The film of invertase on 0·1 N HCl pH 1·0 was transferred to buffer solution pH 3·0 after washing it with distilled water. In the second experiment the film was spread on buffer of pH 3·0 and brought to 0·1 N HCl pH 1·0.
2. Sodium metaphosphate and trichloracetic acid have a contracting effect on the film at low pH but at higher pH the spreading is increased because the tendency of the film to dissolve at higher pH is reduced by these precipitants.
3. Ammonium sulphate at low pH has got a contracting effect but at higher pH this also increases the spreading. Barium chloride however increases the spreading considerably.
4. Invert sugar has been found to have no effect on the spreading of invertase.
5. Heating of invertase makes it completely inactive but the spreading is found to increase.
6. Treatment of the invertase with formaldehyde causes a decrease in spreading.
Volume 36 Issue 5 November 1952 pp 381-387
1. The study of the variation of surface tension with time of invertase solutions has given indication of the possible presence of two types of molecules in invertase.
2. The accumulation is strongly dependent on pH. It appears that this substance becomes strongly hydrophilic at high pH.
3. Formalisation of invertase brings down accumulation perhaps due to polymerisation.
4. A method has been described for the calculation of the order of dimension of the accumulating invertase molecule from the spreading and accumulation data.
Volume 37 Issue 1 January 1953 pp 63-67
A detailed study has been made of the kinetics of inactivation of invertase at 25°C. in acid media. The results indicate the existence of two processes, one of them being very fast and coming up at comparatively high pH values and the other coming up at lower pH.
Volume 38 Issue 3 September 1953 pp 220-225
1. Detergents are shown to decrease the activity of sucrase.
2. The cationic detergents are found to be more effective than the anionic detergents.
3. The critical pH region of inactivation of sucrase is found to shift to higher pH values in the presence of anionic detergents.
4. A provisional explanation based on the Debye ion atmosphere concept has been put forth and the limitations of the hypothesis discussed.
Volume 45 Issue 2 February 1957 pp 91-96
The composition of nickel ferrocyanide was investigated following the amperometric titration of Ni++ up to 40 mM. concentration against potassium ferrocyanide; it appeared to be 3 NiK2 [Fe (CN)6], Ni2 [Fe (CN)6] or Ni5K6 [Fe (CN)6]4. The data showed the possibility of estimating Ni++ polarographically within an experimental error of 1 per cent.
Volume 51 Issue 1 January 1960 pp 34-40
Cadmium was estimated amperometrically using K4Fe (CN)6 as titrating agent, with an experimental inaccuracy of ± 0·5 per cent. In citrates employed as supporting electrolyte for the reduction at d.m.e. of Cd2+, the presence of Ni2+, Co2+ and Bi3+ did not interfere with the estimation, due to the formation of more stable citrate complexes of Ni2+, Co2+ and Bi3+ than the corresponding ferrocyanide complexes.