• H C Saraswat

      Articles written in Proceedings – Section A

    • Formula of complex compounds from solubility data

      H C Saraswat

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      The equation used by Dey for the calculation of the formula of the complex compounds from solubility data is shown to be applicable in cases where either the complex formation is very weak or in cases wheren is equal to unity. In the general case where the complex formation is considerable the graphical method gives more reliable values ofn for the complex formation. The method has been applied to investigate the nature of the complexes formed in the silver phosphate-ammonia water system, the necessary solubility data having been obtained at 35°·5 C.

    • Surface films of invertase

      K S G Doss H C Saraswat

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      1. A new technique has been described for studying the mechanism of action of pH on spreading of invertase. The film of invertase on 0·1 N HCl pH 1·0 was transferred to buffer solution pH 3·0 after washing it with distilled water. In the second experiment the film was spread on buffer of pH 3·0 and brought to 0·1 N HCl pH 1·0.

      2. Sodium metaphosphate and trichloracetic acid have a contracting effect on the film at low pH but at higher pH the spreading is increased because the tendency of the film to dissolve at higher pH is reduced by these precipitants.

      3. Ammonium sulphate at low pH has got a contracting effect but at higher pH this also increases the spreading. Barium chloride however increases the spreading considerably.

      4. Invert sugar has been found to have no effect on the spreading of invertase.

      5. Heating of invertase makes it completely inactive but the spreading is found to increase.

      6. Treatment of the invertase with formaldehyde causes a decrease in spreading.

    • Aging of surfaces of invertase solutions

      H C Saraswat

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      1. The study of the variation of surface tension with time of invertase solutions has given indication of the possible presence of two types of molecules in invertase.

      2. The accumulation is strongly dependent on pH. It appears that this substance becomes strongly hydrophilic at high pH.

      3. Formalisation of invertase brings down accumulation perhaps due to polymerisation.

      4. A method has been described for the calculation of the order of dimension of the accumulating invertase molecule from the spreading and accumulation data.

    • Kinetics of inactivation of invertase

      K S G Doss H C Saraswat

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      A detailed study has been made of the kinetics of inactivation of invertase at 25°C. in acid media. The results indicate the existence of two processes, one of them being very fast and coming up at comparatively high pH values and the other coming up at lower pH.

    • Effect of detergents on the activity and inactivation of invertase

      H C Saraswat

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      1. Detergents are shown to decrease the activity of sucrase.

      2. The cationic detergents are found to be more effective than the anionic detergents.

      3. The critical pH region of inactivation of sucrase is found to shift to higher pH values in the presence of anionic detergents.

      4. A provisional explanation based on the Debye ion atmosphere concept has been put forth and the limitations of the hypothesis discussed.

    • Polarographic studies on the composition of nickel ferrocyanide complex

      H C Saraswat S K D Agarwal

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      The composition of nickel ferrocyanide was investigated following the amperometric titration of Ni++ up to 40 mM. concentration against potassium ferrocyanide; it appeared to be 3 NiK2 [Fe (CN)6], Ni2 [Fe (CN)6] or Ni5K6 [Fe (CN)6]4. The data showed the possibility of estimating Ni++ polarographically within an experimental error of 1 per cent.

    • Polarographic estimation of cadmium in presence of nickel, cobalt and bismuth

      H C Saraswat

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      Cadmium was estimated amperometrically using K4Fe (CN)6 as titrating agent, with an experimental inaccuracy of ± 0·5 per cent. In citrates employed as supporting electrolyte for the reduction at d.m.e. of Cd2+, the presence of Ni2+, Co2+ and Bi3+ did not interfere with the estimation, due to the formation of more stable citrate complexes of Ni2+, Co2+ and Bi3+ than the corresponding ferrocyanide complexes.

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