• Nishant Chakravorty

      Articles written in Resonance – Journal of Science Education

    • Genetic Disorders, Genotyping Techniques and the Emerging Role of Tetra-ARMS-PCR as a Diagnostic Tool

      Motiur Rahaman Mandrita Mukherjee Nishant Chakravorty

      More Details Abstract Fulltext PDF

      Minor deviations in the script of human life (the genetic code) are known to have major consequences on human health and wellbeing. Even substitution of a single nucleotide (single nucleotide polymorphism – SNP) in the gene sequences can cause diseases. SNP genotyping refers to a variety of techniques that allow simultaneous detection of such changes that can be associated with the pathophysiology of different diseases. However, most of the current, cutting-edge SNP geno-typing technologies are complex, expensive, and require post-PCR manipulation. The Tetra–Amplification-Refractory Mutation System–Polymerase Chain Reaction (T-ARMS-PCR) method is a simple tool that meets modern diagnostic labo-ratories’ expectations. It is a fast, reliable, and economical method of genotyping for genetic diseases such as $\beta$-thalassemia.

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