Development of anther tapetum from premeiotic stages to pollen formation was studied in six x-ray induced haploids ofGossypium hirsutum, three interspecific F2 haploids, and one natural haploid of each ofG. hirsutum andG. barbadense, and the observations were compared with those of their respective parents, a genetic male sterile, a male fertile and a cytoplasmic male sterile line ofG. hirsutum. Significant differences were recorded for number of anthers per flower, pollen size, pollen viability and number of microspores produced byPMC. Anther development in haploids was normal. Anther dehiscence was also normal in some haploids. Non-dehiscent anthers could be mostly attributed to the formation of immature pollen grains. Normal development of anthers and degeneration of tapetum occurred in the parents and in the genetic fertile line. Contrastingly no degeneration of tapetum was noticed in the cytoplasmic male sterile line.