• S E Pawar

      Articles written in Proceedings – Plant Sciences

    • Screening for powdery mildew (Erysiphae polygoni DC.) resistance in mungbean (Vigna radiata (L.) Wilczek) using excised leaves

      K S Reddy S E Pawar C R Bhatia

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      A simple, reliable method for assessing powdery mildew reaction on excised mungbean leaves has been developed and used for screening germplasm accessions. Third trifoliate leaves from 21–25 day old field-grown plants were cultured in enamel trays filled with tap water at 21±1°C, 12 h/day photoperiod (4136 lux/m2 from incandescent and white fluorescent light). After 9–12 days such leaves showed rooting from petiole and substantial root growth followed. Excised leaves could be maintained upto 40 days. Leaves were sprayed with conidial suspension (3·5×106 conidia/ml) of a local isolate of powdery mildew fungus. In susceptible types, powdery mildew lesions appeared 8–10 days after inoculation, enlarged and coalesced to cover the entire leaf area in 20 days. Fifty five germplasm accessions were tested using this method; all except 7 RUM accessions were susceptible. These RUM accessions showed resistant reaction even after two reinoculations. They were also resistant in the field under natural powdery mildew infection.

    • Seed coat and hypocotyl pigments in greengram and blackgram

      R N Pandey S E Pawar G J Chintalwar C R Bhatia

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      Three anthocyanins were found inVigna radiata (greengram) accessions with black seed coat, but were absent in cultivars having green seed coat.Vigna mungo (blackgram) had only one of them. The same 3 anthocyanins were found in cultivars with purple red hypocotyl ofVigna radiata accessions but were absent in the ones with green hypocotyls.Vigna mungo with purple red hypocotyls showed the presence of two anthocyanins. One common to both was identified as delphinidin-3-glucoside and the other is probably cyanidin-3-glucoside. Among the two accessions ofVigna radiata varsublobata examined, one was similar toVigna radiata accessions with black seed coat having delphinidin-3-glucoside and two other anthocyanins, while the other resembledVigna mungo having only delphinidin-3-glucoside in seed coat and both delphinidin and cyanidin-3-glucosides in hypocotyl. Chlorophyll content of the seed coats was in the following decreasing order: green, black, brown and yellow. Browning of the seed coat of cv TAP-7 after storage over a year was associated with reduction in chlorophyll content.

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