Stomatal chloroplast count technique as a tool to identify the ploidy status was evaluated in species, hybrids and polyploids (natural and induced) involving the A, B and D genomes inGossypium. The differences between group means were significant. Intra group differences were significant for diploids and hexaploids but this was not due to the genomes involved. Thus some varieties of diploids,G. arboreum (A2) were on par withG. raimondii (D5). The percentage increase in chloroplast number per stoma was 25 for triploids, 72 for tetraploids and 102 for hexaploids taking the diploid number as the base. Ploidyper se had great impact on chloroplast number. It appears that stomatal chloroplast count technique can be used as a rapid method to identify polyploids in the genusGossypium.