• Prem Gupta

      Articles written in Proceedings – Plant Sciences

    • Sulphur amino acids and free amino acids in cotyledons of germinated seedlings ofAlbizzia lebbeck Benth exposed to different light intensities

      Prem Gupta D Mukherjee

      More Details Abstract Fulltext PDF

      Metabolism of sulphur amino acids and free amino acids has been studied in the cotyledons during germination ofAlbizzia lebbeck Benth seeds under different light intensities. Seeds ofA. lebbeck contain a large amount of sulphur amino acids which are rapidly utilised during early stages of germination A similarity in the distribution pattern of amino acids in cotyledon samples raised from different seed stocks is noticed. The level of amino acids was higher in the illuminated samples as compared to dark controls. The higher concentration of asparagine, and SAAII during the seedling growth with the increase in light intensities compared to dark-grown seedlings as well as initial seed sample may indicate the involvement of light-triggered reactions in the synthesis of these metabolites.

    • Changes in proteins, amino and keto acids in different seedling parts ofCyamopsis tetragonolobus Linn. during growth in light and darkness

      Prem Gupta D Mukherjee

      More Details Abstract Fulltext PDF

      Comparative changes in protein, free amino and keto acids have been studied in different seedling parts ofCyamopsis tetragonolobus plants in light and dark. Endosperm recorded higher level of free amino acids in darkness than in light, while a low concentration of protein was exhibited both, in light and dark. The breakdown of soluble protein was more in darkened cotyledon due to higher protease activity. The large increase in the free amino acids in the hypocotyl during seedling growth in the dark may be due to its restricted capacity to incorporate all the amino acids into proteins. Root samples from light recorded higher soluble protein as well as a higher free amino acid pool. α-Oxoglutaric acid (α-oga) was recorded in low levels and at few growth stages in both light and dark. In light raised cotyledon samples, the dominating keto acids are phosphoenolpyruvate and pyruvic acid. Low levels of oxaloacetate in light, like α-oga, indicate its rapid utilization during growth, but its accumulation in the dark may suggest sluggish protein synthesis thus sparing the utilization towards the synthesis of amino acids. Utilization of asparagine and glutamine was also affected in dark.

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