In vitro growth preceded by gamma-radiation was studied in two species with non-localised centromere, namelyLuzula elegans andLuzula pediformis. Seed germination was affected by radiation doses. Cytological investigation revealed dose dependent chromosomal aberration and spindle disturbances. Chromosome break and fragments were present inLuzula elegans but absent inLuzula pediformis. The growth of the callus was affected at higher doses in both the species. The radiosensitivity of the two species ofLuzula with diffuse centromere has been discussed.