I Madhusudana Rao
Articles written in Proceedings – Plant Sciences
Volume 88 Issue 2 March 1979 pp 143-154
Isolation of intact mesophyll cells from the leaves of higher plants
G Rajendrudu I Madhusudana Rao A S Raghavendra V S Rama Das
A total of 146 species of angiosperms belonging to 35 taxonomically diverse families were screened for the isolation of living mesophyll cells from the leaves. Seventy-three species belonging to 22 families, on mild maceration in mortar with the isolation medium (pH 5·8) containing 0·7 M mannitoJ, 2 mM EDTA, 5 mM MgCl2, 5 mM K2HPO4 and 1 mM NaNO3, followed by fractional centrifugation, yielded intact mesophyll cells as seen under a research microscope. The high frequency of cell release, associated with the high percentage recovery of chlorophyll in cells was a common feature of most of the plant species examined by us. Nearly 87% of the chlorophyll present in the leaf could be recovered from the isolated cells in
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