• G Rajendrudu

      Articles written in Proceedings – Plant Sciences

    • Isolation of intact mesophyll cells from the leaves of higher plants

      G Rajendrudu I Madhusudana Rao A S Raghavendra V S Rama Das

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      A total of 146 species of angiosperms belonging to 35 taxonomically diverse families were screened for the isolation of living mesophyll cells from the leaves. Seventy-three species belonging to 22 families, on mild maceration in mortar with the isolation medium (pH 5·8) containing 0·7 M mannitoJ, 2 mM EDTA, 5 mM MgCl2, 5 mM K2HPO4 and 1 mM NaNO3, followed by fractional centrifugation, yielded intact mesophyll cells as seen under a research microscope. The high frequency of cell release, associated with the high percentage recovery of chlorophyll in cells was a common feature of most of the plant species examined by us. Nearly 87% of the chlorophyll present in the leaf could be recovered from the isolated cells inDolichos lablab. The isolated cells retained active photosynthetic carbon metabolism as evidenced by high rates of ferricyanide reduction as well as carbon assimilation.

    • Interspecific differences in the constituents of essential oils ofCymbopogon

      G Rajendrudu V S Rama Das

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      Gas chromatography of essential oils ofCymbopogon showed striking interspecific differences in their major constituents. The two species of lemongrass,C. flexuosus andC. pendulus contained large amounts of citral while the citronella grass species,C. nardus andC. winterianus showed comparable amounts of geraniol, citronellal and citronellol. The essential oil ofC. martini was composed only of geraniol and citronellol and was therefore quite different to the other species. The gas chromatography profiles enable the identification of various species yielding essential oils characterised by their specific constituents.

    • Net photosynthetic rate in relation to leaf anatomical characteristics of C3, C3−C4 and C4 dicotyledons

      A Prasada Rao G Rajendrudu

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      Thirteen dicot weed species comprising C3, C3−C4 and C4 photosynthetic types were examined in an attempt to understand relationships between leaf anatomical characteristics and net photosynthetic CO2 uptake. Plants grown on field soil in open air under natural sunlight were used for measurements of net photosynthetic CO2 uptake and leaf anatomical characteristics. The data on net photosynthetic rates and leaf anatomical features of test plants showed consistent grouping into C3, C3−C4 and C4 photosynthetic types. Overall the C3−C4 group of plants invariably exhibited an intermediate nature in all leaf anatomical characteristics as well as photosynthetic rates between those of C3 and C4 groups of plants.

      Correlation analysis showed significant relationships between photosynthetic rates and some leaf anatomical characteristics across diverse photosynthetic types but these were insignificant among different species of the same photosynthetic type. The results indicate that differences in internal leaf characteristics between different photosynthetic types are probably important factors contributing to differences in their net photosynthetic rates. A definite relationship between photosynthetic capacity to fix CO2 and some leaf anatomical characteristics among diverse photosynthetic type of plants indicate selection for plant species or genotypes with efficient leaf anatomical characteristics could be possible to improve photosynthetic efficiency and, in turn, plant productivity.

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