C R Bhatia
Articles written in Proceedings – Plant Sciences
Volume 96 Issue 2 June 1986 pp 79-112
Current status of the molecular approaches for integrative genetic transformation of plants is reviewed.
Volume 97 Issue 5 October 1987 pp 365-369
A simple, reliable method for assessing powdery mildew reaction on excised mungbean leaves has been developed and used for screening germplasm accessions. Third trifoliate leaves from 21–25 day old field-grown plants were cultured in enamel trays filled with tap water at 21±1°C, 12 h/day photoperiod (4136 lux/m2 from incandescent and white fluorescent light). After 9–12 days such leaves showed rooting from petiole and substantial root growth followed. Excised leaves could be maintained upto 40 days. Leaves were sprayed with conidial suspension (3·5×106 conidia/ml) of a local isolate of powdery mildew fungus. In susceptible types, powdery mildew lesions appeared 8–10 days after inoculation, enlarged and coalesced to cover the entire leaf area in 20 days. Fifty five germplasm accessions were tested using this method; all except 7 RUM accessions were susceptible. These RUM accessions showed resistant reaction even after two reinoculations. They were also resistant in the field under natural powdery mildew infection.
Volume 98 Issue 5 October 1988 pp 405-407
Progenies of androgenetic doubled haploid plants of mustard cv TM-4 in A4−A6 generations were evaluated for their seed yield and oil content. Eight progenies in the A5 generation out of the 97 (8%) A1 plants regenerated were equal in yield to the parent cultivar. The oil percentage in these doubled haploids were in the same range as that of the parent cultivar. The remaining androgenetic doubled haploids were statistically lower in yield. The data indicates that about 8% of the
Volume 99 Issue 4 August 1989 pp 301-306
Three anthocyanins were found in
Volume 100 Issue 1 February 1990 pp 7-10
A true breeding mutant having free corolla lobes, designated as polypetalous mutant was obtained in sesame (