• ZULKIFLI YAAKUB

      Articles written in Journal of Genetics

    • Anchoring a genetic map of an interspecific backcross two family to the genome builds of Elaeis

      KATIALISA KAMARUDDIN MAIZURA ITHNIN1 NGOOT-CHIN TING ZULKIFLI YAAKUB NIK SHAZANA NIK MOHD SANUSI KANDHA SRITHARAN RAJINDER SINGH

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      The present study evaluated an interspecific backcross two (BC$_{2} $) population of oil palm, which was segregating for fatty acidcomposition (FAC). The purpose of this study was to construct a high-density genetic map for the population, which could be used toanchor the Elaeis guineensis (EG5) and E. oleifera (O7) genome builds and determine the physical positions of the quantitative trait loci(QTLs) associated with FAC. A high-density SNP-based and SSR-based linkage map was successfully constructed for an E. guineensis$\times$ E. oleifera BC2 population. The genetic map had 16 linkage groups spanning 1618.4 cM with 1252 markers (1152 SNPs and 100SSRs). The physical location of the markers were determined through sequence similarity search against EG5 and O7. The majority ofmarkers (81.2%) showed map order consistent with their corresponding position on EG5. In total, 1218 markers were also anchored to 683scaffolds in O7. This study for the first time compared the genetic map of the BC$_{2} $ population with that of a published E. oleifera $\times$ E.guineensis (O$\times$G) interspecific F$_{2} $ hybrid, which revealed 433 common markers (34.6%). More importantly marker order was generallyconsistent in both maps. The published EG5 facilitated orientating the mapped markers in the present BC2 map as well as in the O7scaffolds. Major QTLs associated with iodine value (IV) and palmitic acid (C16:0) content, localized on chromosome 3 and linoleic acid(C18:2) content localized on chromosome 2 of EG5 were also for the first time positioned on the O7 build, revealing the correspondingposition in the E. oleifera genome likely influencing FAC in hybrids and backcrosses.

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