• YUUTA KOMATSU

      Articles written in Journal of Genetics

    • Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

      YUUTA KOMATSU SHIN SUKEGAWA MAIYAMA SHITA NAOKI KATSUDA BIN TONG TAKESHI OHTA HIROYUKI KOSE TAKAHISA YAMADA

      More Details Abstract Fulltext PDF

      Suppression subtractive hybridization was used to identify genes showing differential expression profile associated withgrowth rate in skeletal muscle tissue of Landrace weanling pig. Two subtracted cDNA populations were generated from mus-culus longissimus muscle tissues of selected pigs with extreme expected breeding values at the age of 100 kg. Three upregu-lated genes (EEF1A2

      ,TSG101andTTN) and six downregulated genes (ATP5B

      ,ATP5C1

      ,COQ3

      ,HADHA

      ,MYH1andMYH7)in pig with genetic propensity for higher growth rate were identified by sequence analysis of 12 differentially expressed clonesselected by differential screening following the generation of the subtracted cDNA population. Real-time PCR analysis con-firmed difference in expression profiles of the identified genes in musculus longissimus muscle tissues between the two Lan-drace weanling pig groups with divergent genetic propensity for growth rate. Further, differential expression of the identifiedgenes except for theTTNwas validated by Western blot analysis. Additionally, the eight genes other than theATP5C1co-localized with the same chromosomal positions as QTLs that have been previously identified for growth rate traits. Finally,the changes of expression predicted from gene function suggested association of upregulation of expression of theEEF1A2

      ,TSG101andTTNgenes and downregulation of theATP5B

      ,ATP5C1

      ,COQ3

      ,HADHA

      ,MYH1andMYH7gene expressionwith increased growth rate. The identified genes will provide an important insight in understanding the molecular mechanismunderlying growth rate in Landrace pig breed.

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