• XINSHENG WU

      Articles written in Journal of Genetics

    • RNAi-mediated SLC7A11 knockdown inhibits melanogenesis-related genes expression in rabbit skin fibroblasts

      NAISU YANG LIN MU BOHAO ZHAO MANMAN WANG SHUAISHUAI HU BIN ZHAO YANG CHEN XINSHENG WU

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      Solute carrier family 7 member 11 (SLC7A11) is a cystine/glutamate exchanger, also known as xCT, has been found to play an important role in pheomelanin synthesis. Adjusting the cystine content of cells to influence pheomelanin synthesis affects the proportion of total melanin, changing mammalian coat colour. In our previous study, we used RNA-seq to show that SLC7A11 wasinvolved in coat colour regulation in Rex rabbits. However, the precise role of SLC7A11 in rabbit coat colour formation has not been investigated. To better understand the functions of SLC7A11 in rabbits, we used RNA interference (RNAi) to explore the effects of small interfering RNA (siRNA)-mediated downregulation of SLC7A11 gene expression on the expression of melanogenesis-relatedgenes in rabbit skin fibroblasts (RAB-9). The effects of siRNA treatment were measured by quantitative real-time polymerase chain reaction and the efficiency of RNAi was calculated. The expression levels of melanogenesis-related genes, including MITF, MC1R, Agouti, CREB1 and SLC24A5 were detected at 24 h after RNAi transfection. The results showed that MITF, MC1R, SLC24A5, Agouti and CREB1 expression was significantly downregulated after SLC7A11 inhibition. This suggested that changes in SLC7A11 gene expression could directly affect the transcription of genes related to melanin production. This provides a scientific basis for further study of the role of SLC7A11 in the formation of coat colour.

    • Molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-I in duck (Anas platyrhynchos)

      TIANTIAN GU GUOQIN LI YONG TIAN LI CHEN XINSHENG WU TAO ZENG QI XU PYRYDONOV SVLADYSLAV GUOHONG CHEN LIZHI LU

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      Intracellular double-stranded RNA (dsRNA) is a chief sign of replication for many viruses. Pattern recognition receptors(PRRs) of the innate immune system detected the dsRNA and initiate the antiviral responses. Retinoic acid-inducible gene I (RIG-I), a member of PRRs, plays an essential regulatory role in dsRNA-induced signalling. In this study, the full-length complementary DNA (cDNA) of duck RIG-I (duRIG-I) was cloned using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA of duRIG-I contained 97-bp 5'UTR, 141-bp 3'-UTR and 2802 bp complete open-reading frame (ORF) encoding 933 amino acids. Multiple sequence alignments showed that duRIG-I shared high similarity with RIG-I from other vertebrates. Quantitative real-time PCR (qRT-PCR) analysis revealed that duRIG-I mRNA was expressed in all tested tissues, with high levels in the liver, heart, spleen, kidney and thymus, while lower in the duodenum. duRIG-I could be induced by treatment with poly(I:C). Further, overexpression of duRIG-I significantly activated the transcription of poly(I:C)-induced IFN-b, IRF7, TRIF, Mx, STAT1 and STAT2 mRNA, and duRIG-I knockdown showed the opposite results. Overall, our results suggested that duRIG-I could be an important receptor for mimicking antiviral state in duck, which warrant further studies to show the possible mechanism.

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