A number of genetic maps for Fusarium wilt resistance in chickpea have been reported in earlier studies, however QTLs identified for Fusarium wilt resistance were unstable. Hence, the present study aims to map novel molecular markers and to identify QTLs for Fusarium wilt resistance in chickpea. An intraspecific linkage map of chickpea (Cicer arietinum L.) was constructed using F10–F11 recombinant inbred lines (RILs) derived from a cross between K850 and WR315 segregating for H2 locus. A set of 31 polymorphic simple sequence repeat (SSR) markers obtained by screening 300 SSRs and were used for genotyping. The linkage map had four linkage groups and coverage of 690 cM with a marker density of 5.72 cM. The RILs were screened for their wilt reaction across two seasons in wilt sick plot at International Crop Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India. Five major quantitative trait loci (QTLs) were detected in both seasons for late wilting (60 days after sowing). A stable QTL (GSSR 18-TC14801) for wilt resistance was identified in both the seasons, and the QTL explained a variance of 69.80 and 60.80% in 2007 and 2008 rabi respectively.