• Prabodh K. Trivedi

      Articles written in Journal of Genetics

    • ThepsbE-F-L-J operon from chloroplast genome ofPopulus deltoides: Cloning, nucleotide sequence and transcript analysis

      Sushma Naithani Prabodh K. Trivedi Rakesh Tuli Prafullachandra V. Sane

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      A 5-5-kbpHindIII fragment carryingpsbE-F-L-J operon from the chloroplast DNA ofPopulus deltoides was cloned and nucleotide sequence was determined for a 1672-bp region. The coding regions showed more than 90% homology at nucleotide sequence level with plastid-encodedpsbE, psbF, psbL andpsbJ genes of higher plants. Pairwise alignments ofpsbE, psbF, psbL andpsbJ coding regions of poplar with published sequences from other plants were carried out to analyse the nature of nucleotide substitutions. The 5’ and 3’ untranslated regions of the genes revealed high variability among different organisms both in terms of homology and the number of nucleotides. Northern hybridization data indicated that all four genes ofpsbE-F-L-J operon were transcribed as a single tetracistronic message which was not subjected to further processing into smaller transcripts. The transcript showed quantitative increase in mature leaves.

    • Cloning and nucleotide sequence analysis ofpsbD/C operon from chloroplasts ofPopulus deltoides

      M. S. Srinivasa Reddy Prabodh K. Trivedi Rakesh Tuli Prafullachandra V. Sane

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      We report the cloning and nucleotide sequence analysis ofpsbD/C operon from a dicotyledonous tree species,Populus deltoides (poplar). The coding regions ofpsbD andpsbC and deduced amino acid sequences show very high homology with those from other higher plants. In pairwise alignment of the gene sequences,P. deltoides clustered with dicotyledonous annuals rather than withPinus, the only other tree whosepsbD/C nucleotide sequence is available. Comparison of several reported sequences showed that synonymous substitutions were distributed in bothpsbD andpsbC uniformly, throughout the length of the genes. The frequency of nonsynonymous substitutions located in the amino-terminal endof psbD was distinctly higher, suggesting a lower degree of structural constraints in this region of the encoded D2 protein. The arrangement of reading frames and Northern analysis suggest that organization and expression ofpsbD/C operon inP. deltoides is similar to that in other higher plants.

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