Articles written in Journal of Genetics

    • Genetics of resistance to Cercospora leaf spot disease caused by Cercospora canescens and Psuedocercospora cruenta in yardlong bean (Vigna unguiculata ssp. sesquipedalis) × grain cowpea (V. unguiculata ssp. unguiculata) populations


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      Yardlong bean (Vigna unguiculata ssp. sesquipedalis), a type of cowpea, is an important vegetable legume of Asia. Cercospora leaf spot (CLS) caused by Cercospora canescens and Psuedocercospora cruenta is an important phytopathological problem of the yardlong bean grown in tropical regions. The objectives of this study were to (i) determine mode of inheritance of resistance to CLS caused by C. canescens and P. cruenta, (ii) estimate the heritability of the resistance, (iii) estimate genetic effects on the resistance using six basic populations generated from the cross between the susceptible yardlong bean ‘CSR12906’ and the resistant grain cowpea (V.unguiculata spp. unguiculata) ‘IT90K-59-120’. Segregation for the resistance to both fungi in the F2 population fitted both 3 : 1 ratio and 13 : 3 ratio of susceptible:resistant, while that in the BC2 ((CSR12906×IT90K-59-120)×IT90K- 59-120) population fitted a 1 : 1 ratio, suggesting one recessive gene or two genes with inhibitory gene action control the resistance. Generation mean analysis showed that a simple additive–dominance model was adequate to explain the genetic control of CLS disease resistance, indicating that a single gene controls the resistance. The average number of major genes (effective factors) controlling the resistance was estimated to be 1.05 and 0.92 for C. canescens and P. cruenta, respectively. The broad-sense heritability calculated for resistance to both diseases was higher than 0.90. Altogether, these results indicated that the resistance to CLS disease caused by C. canescens and P. cruenta in grain cowpea IT90K-59-120 is a highly heritable trait governed by a single major recessive gene.

    • BADH1 is associated with fragrance in sorghum (Sorghum bicolor (L.) Moench) cultivar ‘Ambemohor’


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      Aroma is an important trait that can enhance the product value in several crops. Pandan-like fragrance resulting fromaccumulation of 2-acetyl-1-pyrroline (2AP) is one of the pleasant aromas in food crops which is caused by null or missense mutations inbetaine aldehyde dehydrogenase 2 (BADH2) gene. In addition, betaine aldehyde aehydrogenase 1 (BADH1) has shown to be associatedwith aroma in rice. In this study, we investigated the genetics controlling coconut juice-like fragrance in inflorescence of sorghum cultivar ‘Ambemohor’. 2AP analysis in seeds revealed that Ambemohor possessed no 2AP. An F$_{2}$ population developed from the cross betweenAmbemohor $\times$ KU630 (nonfragrant) segregated into a ratio of 3 (fragrant) : 1 (nonfragrant), suggesting that the coconut juice-like fragrancein Ambemohor is controlled by a single dominant gene, designated ‘(Aro(’. Bulked segregant analysis suggested that the gene controllingfragrance in Ambemohor is located on sorghum chromosome 6. Quantitative trait locus (QTL) analysis identified a major QTL, (qAro6.1,for the fragrance located on chromosome 6 between markers SB3567 and SB3570. Bioinformatics analysis revealed that SB3567 andSB3570 were 217.8 kb apart and there were 29 annotated genes in this region including BADH1. Sequence analysis revealed that BADH1sequences in Ambemohor and KU630 differed in size, but their coding sequences (CDS) were of same size. CDS alignment revealed foursingle-nucleotide polymorphisms (SNP$_{s}$) between Ambemohor and KU630 in which two SNP$_{s}$ caused amino change in BADH1 of Ambemohor. These results suggested that BADH1 is a candidate gene for the coconut juice-like fragrance in Ambemohor.

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