• NUNUNG HARIJATI

      Articles written in Journal of Genetics

    • First report on DNA content of three species of Amorphophallus

      CHUFENG ZHAO NUNUNG HARIJATI ERXI LIU SURONG JIN YING DIAO ZHONGLI HU

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      The Amorphophallus genus is a perennial herb which belongs to the family Araceae. There are more than 170 species in this genus, which is widely distributed in tropical and subtropical areas. As a kind of food and medicine Amorphophallus has been used for more than 2000 years in China. Because of the high content of konjac glucomannan (KGM) and dietary fiber, it has attracted more attention worldwide. In this article, the DNA contents of A. konjac, A. albus and A. bulbifer in China, A. albus, A. paeoniifolius and A. muelleri in Indonesia were estimated by using flow cytometry. In the samples of China, the DNA contents were 12.95 ± 0.73 pg/2C in A. konjac, 10.51 ± 0.05 pg/2C in A. albus and 17.61 pg/2C in A. bulbifer, and for Indonesia, 14.16 ± 0.48 pg/2C in A. albus (flowering), 8.49 ± 0.2 pg/2C in A. paeoniifolius and 17.84 ± 1.46 pg/2C in A. muelleri were used. Interspecific variation was found significantly (P<0.01), suggesting that DNA content might be a parameter that can be used to differentiate the species. Intraspecific variation has also been found significantly (P<0.01), whether in the same region or between two regions. As far as we know, this is the first report ongenome size estimation of the A. konjac, A. albus and A. muelleri using flow cytometry. Understanding the genome size of Amorphophallus species will help to sequence the genome and analyse the genetic diversity, evolutionary relationship and geographical variation pattern of Amorphophallus species.

    • A mixed ploidy natural population of Amorphophallus muelleri provides an opportunity to trace the evolution of Amorphophallus karyotype

      CHUFENG ZHAO XUAN SHE ERXI LIU NUNUNG HARIJATI TENG TENG YING DIAO SURONG JIN ZHONGLI HU

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      Amorphophallus, a perennial herb belongs to the family Araceae, and is widely distributed in Asia and Africa. As anagricultural crop, it has been cultivated and consumed for ${\sim}$ 2000 years in China. Previous studies have found that there are chromosomenumber and ploidy changes in this genus, but there are a few reports on the evolution of different karyotypes. For this study, we collected 37samples of a wild population of Amorphophallus muelleri from Myanmar and analysed their karyotypes. The karyotype analysis showedthat it is a population with mixed chromosome numbers and ploidy, with four karyotypes of 2n = 24, 26, 28 and 39. Combining the resultsof this study with previous literature, we speculate that karyotypes with 2n = 26 may be the common ancestor, and further the other threekaryotypes were evolved from this by various ways. As far as we know, this is the first attempt to put forward the hypothesis of theevolution of those four karyotypes together. On the other hand, by using inter-simple sequence repeat marker-based unweighted pair groupmethod with arithmetic mean cluster analysis, we found that these individuals of four karyotypes can be divided into four correspondingcategories, indicating that they have been differentiated at the genome, providing a theoretical basis for future use of these wild germplasmresources.

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