• Major Singh

      Articles written in Journal of Genetics

    • Genetic analysis to identify good combiners for ToLCV resistance and yield components in tomato using interspecific hybridization

      Ramesh K. Singh N. Rai Major Singh S. N. Singh K. Srivastava

      More Details Abstract Fulltext PDF

      The interspecific hybridization for tomato leaf curl virus (ToLCV) resistance was carried out among 10 genetically diverse tomato genotypes (diversified by 50 SSR markers). Among the 10 parents, four susceptible cultivars of Solanum lycopersicum were crossed with six resistant wilds, such as S. pimpinellifolium, S. habrochaites, S. chemielewskii, S. ceraseforme, S. peruvianum and S. chilense in a line × tester mating design. All the 24 hybrids and their parents were grown in the field and glasshouse conditions to determine the general-combining abilities (GCA) and specific-combining abilities (SCA). The variances due to SCA and GCA showed both additive and nonadditive gene effects. Based on GCA estimates, EC-520061 and WIR-5032 were good general combiners while based on SCA estimates, PBC × EC-520061 and PBC × EC-521080 were best specific combiners for coefficient of infection and fruit yield per plant in both the environments. These lines could be selected and utilized in ToLCV resistance and high yield breeding programme for improving the traits.

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