• Jagmohan Singh

      Articles written in Journal of Genetics

    • Identification of four genes involved in suppression of the pre-mRNA splicing defect in thesng1-1/rhp6- mutant of fission yeast

      Alpana Naresh Jagmohan Singh

      More Details Abstract Fulltext PDF

      Apart from the global regulators of silencing in the fission yeastSchizosaccharomyces pombe, namelyswi6, clr1,clr2, clr3, clr4 andrik1, the DNA repair generhp6 plays a unique role in mating-type silencing. Recently, we showed thatsng1-1, a mutation in the 5’ splice junction of the second intron of therhp6 gene, leads to derepression of both the silent locimat2 andmat3 in switching background. To address the mechanism ofrhp6 in silencing, we have isolated several extragenic suppressors of thesng1-1/rhp6- mutation. These suppressors fall into four complementation groups and are referred to as suppressor ofrhp6: sur1, sur2, sur3 andsur4. Interestingly, reverse transcriptase polymerase chain reaction analysis of therhp6 transcript shows that in contrast to about <50% level of unsplicedrhp6 pre-mRNA in thesng1-1/rhp6- mutant, there is a restoration of normal splicing to varying degrees in the suppressors. Thesur2 gene belongs to the AAA-ATPase family of proteins, with maximum homology to the SIN1-associated protein SAP1 ofSaccharomyces cerevisiae. We propose thatsur2, along withsur1, sur3 andsur4, may play an as yet uncharacterized role in pre-mRNA splicing.

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