Articles written in Journal of Genetics
Volume 92 Issue 3 December 2013 pp 529-543 Research Article
QTL mapping for combining ability in different population-based NCII designs: a simulation study
Lanzhi Li Congwei Sun Yuan Chen Zhijun Dai Zhen Qu Xingfei Zheng Sibin Yu Tongmin Mou Chenwu Xu Xhongli Hu
The NCII design (North Carolina mating design II) has been widely applied in studies of combining ability and heterosis. The objective of our research was to estimate how different base populations, sample sizes, testcross numbers and heritability influence QTL analyses of combining ability and heterosis. A series of Monte Carlo simulation experiments with QTL mapping were then conducted for the base population performance, testcross population phenotypic values and the general combining ability (GCA), specific combining ability (SCA) and Hmp (midparental heterosis) datasets. The results indicated that:
increasing the number of testers did not necessarily enhance the QTL detection power for GCA, but it was significantly related to the QTL effect.
The QTLs identified in the base population may be different from those from GCA dataset. Similar phenomena can be seen from QTL detected in SCA and Hmp datasets.
The QTL detection power for GCA ranked in the order of DH(RIL) based > F2 based > BC based NCII design, when the heritability was low.
The recombinant inbred lines (RILs) (or DHs) allows more recombination and offers higher mapping resolution than other populations. Further, their testcross progeny can be repeatedly generated and phenotyped. Thus, RIL based (or DH based) NCII design was highly recommend for combining ability QTL analysis. Our results expect to facilitate selecting elite parental lines with high combining ability and for geneticists to research the genetic basis of combining ability.
Volume 94 Issue 2 June 2015 pp 261-270 Research Article
Genomewide mapping reveals a combination of different genetic effects causing the genetic basis of heterosis in two elite rice hybrids
Lanzhi Li Xiaohong He Hongyan Zhang Zhiming Wang Congwei Sun Tongmin Mou Xinqi Li Yuanming Zhang Zhongli Hu
North Carolina design III (NCIII) is one of the most powerful and widely used mating designs for understanding the genetic basis of heterosis. However, the quantitative trait mapping (QTL) conducted in previous studies with this design was mainly based on analysis of variance (ANOVA), composite interval or multiple interval mapping methods. These methodologies could not investigate all kinds of genetic effects, especially epistatic effects, simultaneously on the whole genome. In this study, with a statistical method for mapping epistatic QTL associated with heterosis using the recombinant inbred line (RIL)-based NCIII design, we conducted QTL mapping for nine agronomic traits of two elite hybrids to characterize the mode of gene action contributing to heterosis on a whole genomewide scale. In total, 23 main-effect QTL (M-QTL) and 23 digenic interactions in
Volume 102, 2023
Continuous Article Publishing mode
Click here for Editorial Note on CAP Mode