Palynological investigations are carried out on approximately 538.00-m thick Gondwana strata from borehole SKB-1, Binja Block, South Karanpura Coalfield in Jharkhand. Based on the distribution pattern of age marker palynotaxa, two distinct palynoassemblages are identified. Palynoassemblage-I in the lithologically designated Barren Measures and Barakar formations, between 552.00 and 53.20 m depth show dominance of striate bisaccate (Striatopodocarpites, Crescentipollenites, and Faunipollenites) and abundance of nonstriate bisaccate (Scheuringipollenites). Upward the Palynoassemblage-II (39.50–13.80 m depth) is rich in striate bisaccate (Striatopodocarpites and Crescentipollenites) and significant enveloping monosaccate Densipollenites magnicorpus pollen. These strata have been equated with Raniganj Formation of Latest Permian age. The First Appearance Datum (FAD) of Arcuatipollenites pellucidus, Playfordiaspora cancellosa, Alisporites sp., Falcisporites sp. and Krempipollenites indicus observed at 13.80 and 39.50 m depth, mark the transition of Permian into the Lower Triassic. The FADs of Guttulapollenites spp. at 49.10 and 504.70 m, Goubinispora morondavensis at 415.90 m, Alisporites ovalis and Arcuatipollenites sp. at 526.70 m is observed and suggest that these sediments are equivalent to Raniganj Formation, Late Permian in age.