This study was carried out to evaluate the seasonal variations of seaweed biomass and species composition at six different sites along the coastal areas in Bushehr Province. Sampling depths varied among sites, from 0.3 to 2.0 m below mean sea level. A total of 37 (i.e., 10 Chlorophyta, 12 Phaeophyta and 15 Rhodophyta) seaweed species were collected. Studies were conducted for quantifying the seaweeds during four seasons from October 2008 until July 2009. During present research, Ulva intestinalis and Cladophora nitellopsis of green, Polycladia myrica, Sirophysalia trinodis and Sargassum angustifolium of brown and Gracilaria canaliculata and Hypnea cervicornis of red seaweeds showed highest biomass in coastal areas of Bushehr Province. The Cheney’s ratio of 2.1 indicated a temperate algal flora to this area. All sites exhibited more than 50% similarity of algal species, indicating a relatively homogenous algal distribution. Total biomass showed the highest value of 3280.7 ± 537.8 g dry wt m⁻² during summer and lowest value of 856.9 ± 92.0 g dry wt m⁻² during winter. During this study, the highest and lowest seaweed biomass were recorded on the site 2 (2473.7 ± 311.0 g dry wt m⁻²) and site 5 (856.7 ± 96.8 g dry wt m⁻²), respectively.