• XIN LI

      Articles written in Journal of Chemical Sciences

    • A novel enzymatic biosensor for detection of intracellular hydrogen peroxide based on 1-aminopyrene and reduced graphene oxides

      NA ZOU XIANYONG WEI ZHIMIN ZONG XIN LI ZHAOXIA WANG XUELIANG WANG

      More Details Abstract Fulltext PDF

      An electrochemical sensor for hydrogen peroxide (H2O2) detection was established using 1-aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO) modified glassy carbon electrode. The AP can combine with HRP via a covalent bond and combine withrGO via π-π conjugation. The covalent bond linkages can prevent the enzymes leaching of the enzymes and the π-π conjugate combination can facilitate electrons transfer, which plays synergistic effects to improve the performance of this sensor. The electrochemical activity of the HRP-AP/rGO was described by cyclic voltammetry, alternating current impedance and amperometric techniques. Under the optimal conditions, the biosensor showed a wide linear range from 1.5 μM to 28.5 μM and a low detection limit of 0.5 μM with good stability and high selectivity, confirming that the sensor is well-suited for the detection of H2O2 during biological processes.

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