Articles written in Journal of Chemical Sciences
Volume 131 Issue 10 October 2019 Article ID 0108
A simple method for naked-eye detection of free amine groups during solid-phase peptide synthesis is reported. The technique involves base-catalyzed cyclization of 2-(2-oxo-2H-acenaphthylene-1-ylidene)-malononitrile (1) and subsequent oxidative substitution of aryl moiety by free amine group present atthe N-terminus of the peptide chain that leads to the formation of a chromophore, which distinguishes the deprotected peptide chain from the protected one. The reaction is fast, does not require heating, and allows N-terminal modification of peptides suitable for imaging specific cells.
Volume 134, 2022
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