Synthesis of phenazine derivatives from the reaction of 2-naphthols with 1, 2-diamino benzenes in presence of K₂S₂O₈ in AcOH and water, through the intermediate formation of 1, 2-naphthoquinones from self-coupling of 2-naphthol and then followed by condensation of 1, 2-diamino benzenes in one pot. The present reaction was compatible with various substituted 2-naphthols as well as substituted 1, 2-diaminobenzenes to obtain a variety of substituted phenazine derivatives in good to excellent yields. The reaction was highly regio-selective in the case of unsymmetrical substituted 1, 2-diamino benzenes for providing single regio isomeric phenazine compounds. Reaction conditions were also mild and metal-free and also used green solvents such as AcOH and water. Phenazine derivatives are an important class of heterocycles and occur both in natural and synthetic compounds which shows many biological activities and also present in many important dyestuffs. In meantime, we have also shown our interest in antibacterial, anti-inflammatory activities and molecular docking studies. It is important to note that the phenazine derivatives showed excellent anti-bacterial and anti-inflammatory activities.