A simple Schiff base probe PADP ((1,2-phenylenebis(azanylylidene)) bis(methanylylidene)) bis(3-(diethylamino)phenol) was synthesized from 4-(diethylamino)salicylaldehyde and o-phenylenediamine and has been characterized using analytical and spectral methods. The emission spectrum of PADP Schiff base shows notable enhancement in the emission intensity at 500 nm in the presence of Al³⁺ in MeOH-H₂O (1:9 v/v). On the other hand, other relevant metal ions did not influence considerably the emission spectrum of PADP. The detection limit and binding constant (Kb) of PADP towards Al³⁺ were found to be 0.104 lM and 3.0 9 10⁶ M^-1 from the fluorescence titration experiments. The 1:1 binding stoichiometry was further determined by Job’s plot and ESI-MS spectroscopy. The MTT assay shows no or negligible toxicnature of probe and thus it was utilized in vitro cell imaging studies of Al³⁺ ions. The results show that the probe PADP could be useful to detect Al³⁺ in an aqueous medium