• PRASAD KULKARNI

      Articles written in Journal of Chemical Sciences

    • Cytotoxic properties of metal-quinone complexes: Role of redox cycling in the toxicities of iron(II) and iron(III) complexes of 2- and 5-hydroxy-1,4-naphthoquinones to isolated rat hepatocytes

      Avinash Kumbhar Prasad Kulkarni Subhash Padhye

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    • Photoactivated cytotoxicity induced by heterobimetallic Ru(II)-Pt(II) polypyridyl complexes in MCF-7 cells

      SUSHMA SINGH MOKSHADA VARMA BHUPENDRA SHRAVAGE PRASAD KULKARNI AVINASH KUMBHAR

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      The heterobimetallic Ru(II)-Pt(II) polypyridyl complexes [Ru(bpy)2(BPIMBp)PtCl2]2+ (3) and [Ru(phen)2(BPIMBp)PtCl2]2+ (4) with their parent complexes [Ru(bpy)2BPIMBp]2+(1) and [Ru(phen)2-BPIMBp]2+ (2) possessing bridging ligand (BPIMBp = 1,4ʹ-Bis-{(2-pyridin-2-yl)-1H-imidazol-1-yl)methyl}-1,1ʹ-biphenyl) were used for photocytotoxicity against MCF-7 cells.The parent ruthenium complexes(complexes 1-2) exhibited a negligible increase in viscosity of DNA while heterobimetallic Ru(II)-Pt(II)system exhibited a decrease in viscosity of DNA, indicating covalent interaction (through cis-PtCl2 unit). TheRu(II)-Pt(II) system co-precipitated with CT-DNA confirmed the covalent binding. In electrophoreticmobility studies, the interaction of Ru(II)-Pt(II) system with plasmid DNA led to retardation of negativesupercoiled form, followed by positive supercoiled migration in the dark that indicated the ability to formcovalent adducts with DNA similar to cisplatin. The complexes 1-4 showed enhanced photocleavage ofplasmid DNA on blue light (~450 nm) irradiation. In a cell-based study, all the complexes exhibitedphotoactivated cytotoxicity in MCF-7 cancer cells when exposed to visible light of ~450 nm as compared tolow toxicity in absence of irradiation. Additionally, the complexes containing platinum showed induction ofautophagy in GFP-LC3 expressing MCF-7 cells. Overall our study shows that the inclusion of photosensitizerRu(II) polypyridyl complexes to cis-PtCl2 moiety improves anticancer properties of complexes.

      The effect of Ru(II)-Pt(II) polypyridyl complexes as photosensitizers was studied against MCF-7 cells. The complexes are negligibly toxic to MCF-7 cells in the dark while highly cytotoxic towards MCF-7 cells on irradiation of blue light (~450 nm). Therefore, the complexes could be potential candidates for Photodynamic Therapy.

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