• Kui Jiao

      Articles written in Journal of Chemical Sciences

    • Electrochemical determination of hydrogen peroxide usingo-dianisidine as substrate and hemoglobin as catalyst

      Wei Sun Hong Jiang Kui Jiao

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      A new electrochemical method for the determination of microamounts of hydrogen peroxide utilizing o-dianisidine (ODA) as substrate and hemoglobin (Hb) as catalyst is described in this paper. Hb can be used as mimetic peroxidase and it can catalyse the reduction of hydrogen peroxide with the subsequent oxidation of ODA. The oxidative reaction product is an azo compound, which is an electroactive substance and has a sensitive second-order derivative polarographic reductive peak at the potential of -0.58 V (vs. SCE) in pH 80 Britton-Robinson (B-R) buffer solution. The conditions of Hb-catalytic reaction and polarographic detection of the reaction product were carefully studied. By using this polarographic peak and under optimal conditions, the calibration curve for the H2O2 was constructed in the linear range of 2.0 x 10-7 ∼ 10 x 10-4 mol/l with the detection limit of 5.0 x 10-8 mol/l. This method can also be used to the determination of Hb content in the range of 20 x 10-9 ∼ 30 x 10-7 mol/l with a detection limit of 10 x 10-9 mol/l. The proposed method was further applied to the determination of the content of H2O2 in fresh rainwater with satisfactory results. The catalytic reaction mechanism and the electrode reductive process of the reaction product were carefully studied.

    • Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

      Wei Sun Na Zhao Xueliang Niu Yan Wang Kui Jiao

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      An electrochemical method for the determination of lysozyme (LYS) based on its interaction with alizarin red S (ARS) was established by linear sweep voltammetry in this paper. The electrochemical behaviour of ARS with LYS was investigated on a dropping mercury working electrode in 0.2 mol/L pH 4.8 Britton-Robinson (B-R) buffer solution. ARS showed a sensitive second order derivative linear sweep voltammetric reductive peak at -0.42 V (vs SCE). After the addition of LYS, the reductive peak current of ARS decreased without the shift of the reductive peak potential and no new waves appeared, which was due to the formation of a supramolecular complex of ARS with LYS in the solution. The stoichiometry of the ARS-LYS complex was further calculated by the electrochemical data with the results of the binding ratio as 3 : 1 and the binding constant as $2.82 \times 10^{14}$. Under the selected conditions, the decrease of the second order derivative linear sweep voltammetric reductive peak current of ARS was in proportion to the LYS concentration in the range from 0.8 to 35.0 mg/L and the detection limit of LYS was calculated as 0.52 mg/L (3𝜎). Different kinds of LYS samples were detected satisfactorily with this method.

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