• Jahan-Bakhsh Raoof

      Articles written in Journal of Chemical Sciences

    • Voltammetric sensor for D-penicillamine determination based on its electrocatalytic oxidation at the surface of ferrocenes modified carbon paste electrodes

      Jahan-Bakhsh Raoof Reza Ojani Fereshteh Chekin

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      Electrocatalytic oxidation of D-penicillamine (D-PA) at the surface of ferrocene modified carbon paste electrode (FCCPE) was thoroughly investigated in aqueous solution with various pH. The performance of this modified electrode was compared with those of 2,7-bis(ferrocenyl ethyl) fluoren-9-one modified carbon paste electrode (2,7-BFEFMCPE). In the optimum condition, the oxidation of D-PA at the surface of FCCPE and 2,7-BFEFMCPE is occurred about 480 and 320 mV less positive than that unmodified carbon paste electrode, respectively. The linear dynamic ranges $6 \times 10^{-5}$ M$^{-2} \times 10^{-3}$ M, $6.5 \times 10^{-5}$ M-$1.1 \times 10^{-3}$ M and $7\times 10^{-6}$ M-$1.6 \times 10^{-4}$M, $7 \times 10^{-6}$ M-$2 \times 10^{-4}$ M of D-PA are obtained from CV and DPV methods for FCCPE and 2,7-BFEFMCPE, respectively. The detection limits (3𝜎) were determined as $5.4 \times 10^{-5}$ M and $6.3 \times 10^{-5}$ M in CV and $6.2 \times 10^{-6}$ M and $6.8 \times 10^{-6}$ M in DPV determinations for FCCPE and 2,7-BFEFMCPE, respectively. The proposed method was applied in a highly sensitive determination of D-PA in drug and human synthetic serum samples by standard addition and recovery methods, respectively.

    • A bimetallic nanocomposite electrode for direct and rapid biosensing of p53 DNA plasmid

      Ezat Hamidi-Asl Jahan-Bakhsh Raoof Nahid Naghizadeh Simin Sharifi Mohammad Saeid Hejazi

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      A new label-free electrochemical DNA biosensor is presented based on carbon paste electrode (CPE) modified with gold (Au) and platinum (Pt) nanoparticles to prepare the bimetallic nanocomposite electrode. The proposed sensor was made by immobilization of 15-mer single stranded oligonucleotide probe related to p53 gene for detection of DNA plasmid samples. The hybridization detection relied on the alternation in the guanine oxidation signal following hybridization of the probe with complementary genomic DNA.The technique of differential pulse voltammetry (DPV) was used for monitoring guanine oxidation. To optimize the performance of the modified CPE, different electrodes were prepared in various percentages of Au and Pt nanoparticles. The modified electrode containing 15% Au/Pt bimetallic nanoparticles (15% Au/Pt-MCPE) was selected as the best working electrode. The selectivity of the sensor was investigated using plasmid samples containing non-complementary oligonucleotides. The detection limit of the biosensor was studied and calculated to be 53.10 pg 𝜇L−1.

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