• G V Rajendrakumar

      Articles written in Journal of Chemical Sciences

    • Chemical synthesis of d(G-C)4, d(G-C)5 and d(GGTGGACCTC) by continuous flow solid phase phosphotriester method

      G V Rajendrakumar N Sivakama Sundari K N Ganesh

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      A simplified protocol for the synthesis of oligodeoxyribonucleotides by phosphotriester approach on controlled pore glass resins using a manualdna synthesiser is presented. The main features of this method are: (i) a single system of solvents (acetonitrile:dichloromethane, 8∶2) is used in the assembly procedure reducing the number of mechanical manipulations, (ii) dichloroacetic acid is used as a good compromise between the efficiency of deprotection and minimal depurination and (iii) it competes effectively with the phosphite method in terms of speed, efficiency and ease. All the required protected mononucleotides and functionalised resins were home-made and detailed procedures are reported. The utility of the procedure is demonstrated by the actual synthesis of sequences d(G-C)4, d(G-C)5 and d(GGTGGACCTC) required for biophysical studies in our laboratory. The oligonucleotides were purified by the recently introduced method of fast protein liquid chromatography which gives good resolutions in shorter time periods as compared to the high performance liquid chromatography technique.

    • Rapid chemical synthesis of d(CACGTG) in milligram amounts by solution-phase phosphotriester chemistry

      G V Rajendrakumar N Sivakamisundari K N Ganesh

      More Details Abstract Fulltext PDF

      This paper reports an optimised method for chemical synthesis of short oligodeoxyribonucleotides in milligram amounts by solution-phase phosphotriester chemistry. The procedure is a slight modification of one reported earlier in the literature. Detailed experimental procedures for condensation, deprotection and purification are described. The efficiency of the method is demonstrated by the synthesis of a hexamer, d(CACGTG), in good yields and high purity. The homogeneity of the product and the sequence composition are shown by 270 MHz1H NMR spectrum.

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