In this study, derivatives of new vitamin K3 were synthesized by the reactions of 2-methyl-1,4-naphthoquinone 1 with some heterocyclic ring substituted nucleophiles: 1-piperonylpiperazine 2, 1-(2-furoyl)piperazine 5, 1-(2-aminoethyl)piperidine 8, 1-(2-aminoethyl)pyrrolidine 10 and 2,6-dimethyl morpholine 12 in chloroform/triethylamine (TEA) or ethanol at room temperature. Their structures were characterized by Fourier transform infrared spectroscopy (FT-IR), ¹H nuclear magnetic resonance (¹H NMR),attached proton test nuclear magnetic resonance (APT-NMR) and mass spectrometry (MS). Newly synthesizedvitamin K3 derivatives (3, 4, 6, 7, 9, 11, 13, 14) have shown catalase inhibition activity and compound 13 has displayed remarkable potency against catalase enzyme. These compounds were also tested for their antioxidant capacity in vitro by CUPRAC method