A Highly efficient and environmentally friendly route for the one-pot multi-component construction of biologically active pyrazolopyranopyramidine derivatives by the reaction of aromatic aldehydes,hydrazine hydrate, ethyl acetoacetate, and barbituric acid under ultrasound has been evolved. The reaction ispromoted by starch@Fe₃O₄ at room temperature using water as solvent. The key features of the presentreaction are the excellent yield of products, mild reaction condition, without column chromatography,reusability, and magnetically separable catalyst. The characterization of prepared catalyst and products were performed by XRD, Fourier transforms infrared spectra (FT-IR), thermal analysis (TGA), TEM, FE-SEM,EDAX, vibrating sample magnetometer (VSM) techniques, melting point, ¹³CNMR, and ¹HNMR analysis.The obtained products (5a–5l) were screened for antimicrobial activity. According to results, synthesized compounds exhibited potent antimicrobial activities against fungi C. albicans, Gram-positive bacteria S.aureus and S. phyogens, and Gram-negative bacteria E. coli and P. aeruginosa.

A highly efficient and environmentally friendly route for the one-pot multi-component construction of biologically active pyrazolopyranopyramidine derivatives by the reaction of aromatic aldehydes, hydrazine hydrate, ethyl acetoacetate, and barbituric acid under ultrasonication has been evolved. The reaction is promoted by starch@Fe₃O₄ at room temperature using water as solvent. The key features of the present reaction are the excellent yield of products, mild reaction condition, without column chromatography, reusability, and magnetically separable catalyst.