• Beena Mishra

      Articles written in Journal of Chemical Sciences

    • Fluorescence spectroscopic studies on binding of a flavonoid antioxidant quercetin to serum albumins

      Beena Mishra Atanu Barik K Indira Priyadarsini Hari Mohan

      More Details Abstract Fulltext PDF

      Binding of quercetin to human serum albumin (HSA) was studied and the binding constant measured by following the red-shifted absorption spectrum of quercetin in the presence of HSA and the quenching of the intrinsic protein fluorescence in the presence of different concentrations of quercetin. Fluorescence lifetime measurements of HSA showed decrease in the average lifetimes indicating binding at a location, near the tryptophan moiety, and the possibility of fluorescence energy transfer between excited tryptophan and quercetin. Critical transfer distance (Ro) was determined, from which the mean distance between tryptophan-214 in HSA and quercetin was calculated. The above studies were also carried out with bovine serum albumin (BSA).

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