Yaohua Ji
Articles written in Journal of Biosciences
Volume 32 Issue 6 September 2007 pp 1111-1118 Articles
Human cytomegalovirus
Zhengrong Sun Ying Lu Qiang Ruan Yaohua Ji Rong He Ying Qi Yanping Ma Yujing Huang
Human cytomegalovirus (HCMV), a ubiquitous human pathogen, is the leading cause of birth defects in newborns. A region (referred to as UL/b′) present in the Toledo strain of HCMV and low-passage clinical isolates) contains 22 additional genes, which are absent in the highly passaged laboratory strain AD169. One of these genes,
Volume 35 Issue 3 September 2010 pp 365-370 Articles
Transcription pattern of
Zhengrong Sun Gaowei Ren Yanping Ma Ning Wang Yaohua Ji Ying Qi Mali Li Rong He Qiang Ruan
Human cytomegalovirus (HCMV) mRNA was obtained from human embryonic lung fibroblast cells infected by HCMV clinical strains from urine samples of infants at different kinetic periods. The cDNA of
Volume 38 Issue 3 September 2013 pp 479-485 Articles
Yujing Huang Ying Qi Yanping Ma Rong He Yaohua Ji Zhengrong Sun Qiang Ruan
MicroRNAs (miRNAs) are small RNAs, 19–23 nucleotides in length, which regulate a variety of cellular processes. Human cytomegalovirus (HCMV) encodes only one intronic miRNA: human cytomegalovirus microRNA UL36 (hcmv-miR-UL36). In this study, we found that over-expression of hcmv-miR-UL36 resulted in a more than threefold increase in HCMV DNA synthesis at 24 h post infection. Fifteen putative targets of hcmv-miR-UL36 were identified using hybrid PCR, one being the HCMV UL138 gene that has previously been identified as a novel latency-associated determinant of HCMV infection. Down-regulation of UL138 expression by hcmv-miR-UL36 was validated using luciferase reporter assays and Western blot analysis in HEK293 cells. In the presence of hcmv-miR-UL36, we observed a 74.6% decrease in luciferase activity and a 46.2% decrease in HCMV UL138 protein expression. Our results indicate that hcmv-miR-UL36 may be a viral miRNA contributing to HCMV replication.
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