Articles written in Journal of Biosciences
Volume 44 Issue 6 December 2019 Article ID 0136 Article
To elucidate one mechanism by which putrescine (Put) functions in plant signaling under osmotic stress, Put and ABAcontents, and plasma membrane-NADPH oxidase (PM-NOX) activity were detected in wheat seedling leaves. Underosmotic stress, ABA and Put contents, PM-NOX activity, and PM-NOX-dependent O2.- production all increased. Theinhibitor tungstate (T) of ABA bio-synthesis reduced the increases in ABA and Put contents under osmotic stress. Theinhibitor D-arginine (D-Arg) of Put bio-synthesis didn’t reduce osmotic-induced increase of ABA, but it inhibited theincreases of PM-NOX activity and O2 . - production, and the inhibitory effects were reversed by exogenous Put. Thesefindings suggested that ABA might regulate Put biosynthesis, and Put might regulate PM-NOX activity. Treatments withthree inhibitors imidazole (I), diphenylene iodonium (DPI) and pyridine (P) of PM-NOX reduced significantly not only O2 . -production, but also the stress-induced increase of Put content, which indicated that O2 . - production might regulate Putbiosynthesis. Treatments with EGTA (Ca2+ chelator), La3+ and verapamil (V) (Ca2+ channel blockers) reduced significantlythe stress-induced increase of Put content, which suggested that Ca2+ might regulate Put biosynthesis. With thesefindings, it could be concluded that Put was involved in ABA signaling induced by osmotic stress via regulating PM-NOXactivity in wheat seedling leaves.
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