Articles written in Journal of Biosciences

    • An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton

      Yingfan Cai Hong Zhang Yu Zeng Jianchuan Mo Jinku Bao Chen Miao Jie Bai Fang Yan Fang Chen

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      A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm x 250 mm, 5 Μm particle) with methanol-0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. Absolute recoveries were > 94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glandedGossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton

    • Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities


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      The study was designed to explore the beneficial effect of Musca domestica larvae extract (MDLE) on a metabolic disorderusing a diabetic rat model. Streptozotocin-induced diabetic rats were treated with or without MDLE. Blood glucose, insulinlevels, lipid profiles, and oxidative stress markers were measured. The morphological changes in the pancreas and liverwere determined, as well as insulin expression. The expression of glucose transporter 4 (GLUT4), phospho-adenosinemonophosphate-activated protein kinase (p-AMPK)/total AMPK, superoxide dismutase 1 (SOD1), catalase (CAT), andperoxisome proliferator-activated receptor gamma (PPAR𝛾) were detected. Compared with untreated diabetic rats, MDLEtreatedrats had decreased urine volume, food intake, and water intake, along with significantly lower levels of bloodglucose, malondialdehyde (MDA), plasma triglycerides, low-density lipoprotein (LDL), and total cholesterol. MDLE-treatedrats also had higher levels of SOD activity, high-density lipoprotein (HDL), and insulin. MDLE treatment partiallyrestored the β-cell population, improved the liver necrosis and islet cell damage, reversed the decreased expression ofGLUT4, phospho-AMPK, SOD1, and CAT in the liver, skeletal muscle and pancreatic tissue, and also increased theexpression of PPAR𝛾 in the liver and adipose tissue in diabetic rats. In conclusion, the obtained results suggest that MDLEcould possibly be used pharmacologically as an adjuvant for the treatment of diabetes.

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