The study was designed to explore the beneficial effect of Musca domestica larvae extract (MDLE) on a metabolic disorderusing a diabetic rat model. Streptozotocin-induced diabetic rats were treated with or without MDLE. Blood glucose, insulinlevels, lipid profiles, and oxidative stress markers were measured. The morphological changes in the pancreas and liverwere determined, as well as insulin expression. The expression of glucose transporter 4 (GLUT4), phospho-adenosinemonophosphate-activated protein kinase (p-AMPK)/total AMPK, superoxide dismutase 1 (SOD1), catalase (CAT), andperoxisome proliferator-activated receptor gamma (PPAR𝛾) were detected. Compared with untreated diabetic rats, MDLEtreatedrats had decreased urine volume, food intake, and water intake, along with significantly lower levels of bloodglucose, malondialdehyde (MDA), plasma triglycerides, low-density lipoprotein (LDL), and total cholesterol. MDLE-treatedrats also had higher levels of SOD activity, high-density lipoprotein (HDL), and insulin. MDLE treatment partiallyrestored the β-cell population, improved the liver necrosis and islet cell damage, reversed the decreased expression ofGLUT4, phospho-AMPK, SOD1, and CAT in the liver, skeletal muscle and pancreatic tissue, and also increased theexpression of PPAR𝛾 in the liver and adipose tissue in diabetic rats. In conclusion, the obtained results suggest that MDLEcould possibly be used pharmacologically as an adjuvant for the treatment of diabetes.