Articles written in Journal of Biosciences
Volume 42 Issue 4 December 2017 pp 575-584 Article
MicroRNA-486-5p suppresses TGF-b2-induced proliferation, invasion and epithelial–mesenchymal transition of lens epithelial cells by targeting Smad2
BEI LIU JIANHUA SUN XIAOQIN LEI ZHONGQIAO ZHU CHENG PEI LI QIN
The pathological development of lens epithelial cells (LECs) leads to posterior capsular opacification (PCO). This studywas undertaken to investigate the effects of microRNA-486-5p (miR-486-5p) on TGF-b2-induced proliferation, invasionand epithelial-mesenchymal transition (EMT) in the lens epithelial cell line SRA01/04, and to explore the underlyingmolecular mechanisms. The expression of miR-486-5p in TGF-b2-induced SRA01/04 cells was down-regulated, and theexpression of Smad2, p-Smad2 and p-Smad3 was up-regulated. A dual-luciferase reporter assay revealed that miR-486-5pdirectly targets the 30-UTR of Smad2. MiR-486-5p mimic transfection markedly down-regulated the expression levels ofSmad2, thus inhibiting the expression of p-Smad2 and p-Smad3. MiR-486-5p overexpression in SRA01/04 cells markedlysuppressed TGF-b2-induced proliferation and invasion, inhibited protein expression of CDK2 and CDK4, down-regulatedfibronectin, a-SMA and vimentin and up-regulated E-cadherin; these effects were partly reversed by Smad2 overexpression.In short, these data show that miR-486-5p overexpression can inhibit TGF-b2-induced proliferation, invasion andEMT in SRA01/04 cells by repressing Smad2/Smad3 signalling, implying that miR-486-5p may be an effective target tointerfere in the progression of PCO.
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