Articles written in Journal of Biosciences
Volume 42 Issue 4 December 2017 pp 637-645 Article
Pneumonia is an inflammatory condition affecting the lungs, in which pro-inflammatory cytokines are secreted. It has beenshown that microRNA-146 (miR-146) is involved in the regulation of immune and inflammatory responses. The presentstudy explored the protective effects of miR-146 overexpression on lipopolysaccharide (LPS)-mediated injury in A549 andH1975 cells. In this study, A549 and H1975 cells were transfected with miR-146 mimic or inhibitor, and then weresubjected with LPS. Thereafter, cell viability, colony formation capacity, apoptosis, the release of proinflammatory factors,Sirt1 expression, and the expression of NF-kB and Notch pathway proteins were respectively assessed. As a result, miR-146 overexpression exerted protective functions on LPS-damaged A549 and H1975 cells, as evidenced by the increases incell viability and colony number, the decrease in apoptotic cell rate, as well as the down-regulations of IL-1, IL-6, and TNFa.Sirt1 can be positively regulated by miR-146. Furthermore, miR-146 overexpression blocked NF-kB and Notch pathways,while these blocking effects were abolished when Sirt1 was silenced. The findings in the current study indicated thatmiR-146 protected A549 and H1975 cells from LPS-induced apoptosis and inflammation injury. miR-146 exerted protectivefunctions might be via up-regulation of Sirt1 and thereby blocking NF-kB and Notch pathways.
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