• Vanita A Padgaonkar

      Articles written in Journal of Biosciences

    • Characterisation of lactic dehydrogenase fromLactobacillus casei

      Vanita A Padgaonkar S F D’souza G B Nadkarni

      More Details Abstract Fulltext PDF

      Lactic dehydrogenase fromLactobacillus casei ATCC 7469 has been purified to homogeneity by a two-step affinity chromatography procedure which gave an yield of 35%. The enzyme specifically catalysed the conversion of pyruvate to lactate. The enzyme was maximally active at pH 4.6, which was shifted to 5.4 in the presence of fructose 1,6-biphosphate. The enzyme had a molecular weight of 70,800 with two identical subunits, unlike the lactic dehydrogenase from other sources. Histidine and primary amino groups appeared to be involved in catalysis.

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