• VINCENZO IERARDI

      Articles written in Journal of Biosciences

    • Klebsiella pneumoniae antibiotic resistance identified by atomic force microscopy

      VINCENZO IERARDI PAOLO DOMENICHINI SILVIA REALI GIAN MARCO CHIAPPARA GIANLUIGI DEVOTO UGO VALBUSA

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      In the last decade the detection of the resistance of bacteria to antibiotics treatment, developed by different kind of bacteria,is becoming a huge problem. We hereby present a different approach to the current problem of detection of bacteriaresistance to antibiotics. Our aims were to use the atomic force microscopy (AFM) to investigate bacteria morphologicalchanges in response to antibiotics treatment and explore the possibility of reducing the time required to obtain informationon their resistance. In particular, we studied Klebsiella pneumoniae bacteria provided by the Lavagna Hospital ASL4Liguria (Italy), where there are cases linked with antibiotics resistance of the Klebsiella pneumoniae. By comparing AFMimages of bacteria strains treated with different antibiotics is possible to identify unambiguously the Klebsiella pneumoniaestrains resistant to antibiotics. In fact, the analysis of the AFM images of the antibiotic-sensitive bacteria shows clearly thepresence of morphological alterations in the cell wall. While in the case of the antibiotic-resistant bacteria morphologicalalterations are not present. This approach is based on an easy and potentially rapid AFM analysis.

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