• V Sitaramam

      Articles written in Journal of Biosciences

    • Definition of physical integrity of synaptosomes and myelosomes by enzyme osmometry

      V Sitaramam M K Janardana Sarma

      More Details Abstract Fulltext PDF

      Isotonic requirements for synaptosomes were shown to vary with the concentration of sucrose or mannitol in the isolation medium, as well as with their differential permeability to polyols and ions. The technique of enzyme osmometry, which permits quantitation of the osmotic integrity in a heterogeneous population, was used to defined the osmotic requirements for synaptosomes and myelosomes in a variety of ionic and non-electrolyte media. Important differences, observed in the rank order of permeability of synaptosomal and myelosomal membranes to electrolyte media, were consistent with the known channel density/electrical activity of the corresponding plasma membranes.

    • Erythrocyte stability under imposed fields

      V Sitaramam

      More Details Abstract Fulltext PDF

      Lysis of erythrocytes offers an unique opportunity to probe the fine structure of the bilayer as a function of its state of energization. Critical monitoring of the volumes, ion fluxes and related measures in erythrocytes exposed to a variety of milieu and treatments showed that one can critically distinguish the nature of the prelytic perturbations and the proximate forces actually responsible for the disruption of the membranes among surface charge density, elastic energy etc.

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