Articles written in Journal of Biosciences
Volume 1 Issue 3 September 1979 pp 307-316
RNA extracted from purified rinderpest virus was characterised by sucrose gradient sedimentation and polyacrylamide gel electrophoresis. The predominant virion RNA species had a sedimentation constant of 46S and its estimated molecular weight was 4.8 × 106 daltons. Consistently high amounts of UMP and AMP were detected. The melting-temperature profile of the virion RNA suggested absence of secondary structure.
The effect of actionomycin D on the replication of rinderpest virus in Vero cells was studied by following the viral RNA synthesis using labelled uridine as well as by infectivity titration. The viral RNA synthesis was not affected until 12 h following infection and was inhibited thereafter between 18 and 48 h to an extent of 25% at 5 and 10 Μg levels of the drug. A 100 to 1000-fold reduction in the infectivity titres was observed in the presence of the drug. These results suggest that actinomycin D inhibits rinderpest viral RNA replication. Sedimentation analysis of viral RNA extracted from drug-treated cultures showed inhibition of the genome RNA of rinder-pest virus.
Volume 1 Issue 3 September 1979 pp 327-334
Volume 5 Issue 4 December 1983 pp 347-353
Isolated nuclei from differentiating cultures of
Volume 6 Issue 2 June 1984 pp 213-220
Volume 6 Issue 5 December 1984 pp 757-770
Transfer RNA is uniquely enriched with modified bases. A large body of information has accumulated about the occurrence, nature and distribution of modified bases in tRNA. But similar investigations on the enzymes involved in this post-transcriptional modification have been hampered by the instability of the enzymes and lack of suitable substrates. The present review summarises briefly, the occurrence and methods of detection of modified bases, the enzymes involved in their formation and also certain suggestive evidence for the role of modification in cellular metabolism.
Volume 9 Issue 1-2 September 1985 pp 47-52
N-(2-naphthyl)glycine hydrazide and N-methyl-N-(2-naphthyl) glycine hydrazide, which inhibit
Volume 17 Issue 3 September 1992 pp 305-312
McGlynn and her co-workers have reported that among the Vietnamese refugees in Philadelphia and among Alaskan natives who are hepatitis B carriers, there is a statistically significant association between a
Volume 24 Issue 2 June 1999 pp 143-152 Perspectives