T Rajendrakumar
Articles written in Journal of Biosciences
Volume 5 Issue 4 December 1983 pp 355-363
On the presence of sulphate in pituitary lutropin
A S Arunasmithasri T Rajendrakumar K Muralidhar
The presence of sulphate in the carbohydrate of pituitary lutropin from different species has been investigated using a biosynthetic approach. Pituitaries from rats, rabbits, goats, and buffaloes were incubated in the presence of35SO4- and the35SO4- -labelled proteins in the tissue immunoprecipitated with a well characterized anti-sheep lutropin serum. The incorporation into immunoreactive lutropin was low in the case of rat, rabbit and goat pituitaries while, it was considerable in the case of buffalo pituitaries. Hence further characterization studies were carried out on35SO4- -labelled proteins of buffaloes. The physico-chemical, immunological and biological properties of radio-labelled buffalo pituitary material were shown to be similar to those of standard lutropin. In
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