• T J Pandian

      Articles written in Journal of Biosciences

    • Construction, electroporatic transfer and expression of ZpβypGH and ZpβrtGH in zebrafish

      S G Sheela J D Chen S Mathavan T J Pandian

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      Recombinant transformation vectors (ZPβypGH and ZpβrtGH) consisting of fish growth hormone cDNA, and a reporter geneβ-galactosidase driven by fish promoter (Zp) were constructed. Freshly fertilized eggs of zebrafish (Brachydanio rerio) were electroporated at optimum conditions (0.07 kV voltage; 25 μF capacitance; 8 ohm resistance and 2 pulses) in the presence of one of these transformation vectors (100 μg circular DNNml). In either cases 72% of the electroporated eggs successfully hatched, in comparison to the 85% hatchability of the control eggs. Genomic DNA extracted from fins of randomly chosenF0 individuals was screened (by Southern blot hybridization); the transgenes were retained in the host genome of all the randomly chosen adult transformants. Fin-positive presumptive founder parents were crossed with control counterparts and the DNA of randomly chosenF1 progenies was screened for germline transformation. Southern analysis of chosenF1 progenies revealed the persistence of ZPβypGH or ZpβrtGH in 53% of theF1 progenies. Southern analyses of chosenF1 progenies and the frequency (53% ofF1 ZpβrtGH and 53% ofF1 ZP{β}ypGH) of transmission revealed the degree of mosaicism inF0 transformants. Expression was confirmed from the 3–4 times elevated levels of activity of the reporter gene and 30–40% accelerated growth of transgenicF0 andF1 progenies.

    • Cloning of partial putative gonadotropin hormone receptor sequence from fish

      G Kumaresan T Venugopal A Vikas T J Pandian S Mathavan

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      A search for the presence of mariner-like elements in theLabeo rohita genome by polymerase chain reaction led to the amplification of a partial DNA sequence coding for a putative transmembrane domain of gonadotropin hormone receptor. The amplified DNA sequence shows a high degree of homology to the available turkey and human luteinizing and follicle stimulating hormone receptor coding sequences. This is the first report on cloning such sequences of piscine origin.

    • Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

      T Venugopal S Mathavan T J Pandian

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      A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96–98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

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