• Sharmila Chattopadhyay

      Articles written in Journal of Biosciences

    • Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid

      Riddhi Datta Ragini Sinha Sharmila Chattopadhyay

      More Details Abstract Fulltext PDF

      Salicylic acid (SA) has been implicated in determining the outcome of interactions between many plants and their pathogens. Global changes in response to this phytohormone have been observed at the transcript level, but little is known of how it induces changes in protein abundance. To this end we have investigated the effect of 1 mM SA on soluble proteins of Arabidopsis thaliana leaves by proteomic analysis. An initial study at transcript level has been performed on temporal landscape, which revealed that induction of most of the SA-responsive genes occurs within 3 to 6 h post treatment (HPT) and the expression peaked within 24 HPT. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF MS/MS analysis has been used to identify differentially expressed proteins and 63 spots have been identified successfully. This comparative proteomic profiling of SA treated leaves versus control leaves demonstrated the changes of many defence related proteins like pathogenesis related protein 10a (PR10a), disease-resistance-like protein, putative late blight-resistance protein, WRKY4, MYB4, etc. along with gross increase in the rate of energy production, while other general metabolism rate is slightly toned down, presumably signifying a transition from ‘normal mode’ to ‘defence mode’.

    • Crosstalk between MAPKs and GSH under stress: A critical review


      More Details Abstract Fulltext PDF

      Plants are frequently exposed to a plethora of unfavourable climatic catastrophes, be it abiotic or biotic stresses, viz., salinity, water (drought or water logging), extreme temperature, heavy metal, nutrient deficiency, ozone, pathogen attack, etc., which badly affect the yield and productivity of crops. Plants, as part of their defence machinery, employ different tolerance mechanisms to survive under adverse conditions. In addition to other stress responses, the mitogen-activated protein kinase (MAPK) signalling cascade and accumulation of glutathione (GSH) are two important aspects of plant defence response. Induction of the MAPK cascade is one of the earliest responses when a plant is under any environmental stress, and there is documentary evidence of this signalling pathway, in turn, regulating various phytohormone-signalling networks and other defence-related pathways during stress. Similarly, GSH being a low molecular weight metabolite also has a key role in environmental stress tolerance. It is known to be involved in multi-step interactions with various phytohormones, many signalling molecules, and redox molecules such as reactive oxygen species (ROS). This review provides an outline on GSH–MAPK crosstalk to better understand its role in the context of defence signalling in plants.

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    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".

      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.

      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.

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      Posted on April 12, 2023
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