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Shalini Gulati
Articles written in Journal of Biosciences
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Volume 24 Issue 1 March 1999 pp 59-67 Articles
Platelet Ca2+ handling in essential hypertension: Role of a plasma ouabain-like factor
Shalini Gulati Madhu Khullar Ramandeep Singh Savita Kumari N K Ganguly B K Sharma
A humoral ouabain-like plasma factor has been observed in patients with essential hypertension (EHT). In the present study, we hypothesized that this humoral factor might be responsible for the elevated cytosolic free calcium concentrations [Ca2+]i seen in these patients. Patients with mild to moderate EHT and their normotensive first degree blood relatives (NTBR) participated in the study. Platelet Na+, K+-ATPase activity was assayed in EHT patients and their NT first-degree relatives. To confirm the ouabain-like activity in plasma from EHT patients, control platelets were incubated with EHT and NTBR plasma and their Na+, K+-ATPase activity was measured. In addition, the effect of EHT plasma on platelet45Ca-uptake was studied. The
in vitro effects of ouabain (10 ΜM) on (i)45Ca-uptake and (ii) [Ca2+]i response in control platelets were also observed. A decreased Na+K+-ATPase activity (P < 0.05) was observed in platelet membranes from EHT patients. Incubation of control platelets with EHT plasma decreased their Na+, K+-ATPase activity (P < 0.01) and increased their45Ca-uptake (P < 0.05). C-18 Sep-Pak filtered hypertensive plasma extracts (containing the ouabain-like fraction) also decreased Na+, K+-ATPase activity (P < 001) in control platelet membranes.In vitro incubation of control platelets with ouabain increased45Ca-uptake (P < 005) and [Ca2+]i response (P < 0.05) in these platelets. Thus it appears that an ouabain-like factor in the EHT plasma may contribute to the elevated platelet [Ca2+]i observed in EHT patients.
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Journal of Biosciences
Volume 48, 2023
All articles
Continuous Article Publishing mode
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Journal of Biosciences | News
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To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
Posted on April 12, 2023< -
Editorial Note on Continuous Article Publication
Posted on July 25, 2019Click here for Editorial Note on CAP Mode
Forthcoming Special issue.
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