Articles written in Journal of Biosciences
Volume 9 Issue 3-4 December 1985 pp 159-163
The ontogeny of hexokinase, phosphofructokinase, phosphoglucoisornerase, aldolase, pyruvate kinase and lactate dehydrogenase activities which are associated with glycolysis, an important energy yielding process, has been studied in human fetal heart for periods ranging from 13 weeks to above 33 weeks of gestation. Hexokinase, phosphoglucoisomerase and pyruvate kinase activities show similar developmental profiles exhibiting maximum activity at 25–28 weeks ofgestation. Phosphofructokinase activity, on the other hand, shows a minimum at this period and exhibits a peak value at early stages (13–16 weeks of gestation). Though considerable activity for aldolase is observed at an early period, it declines thereafter, but again increases in the later period. The probable role and correlations of these glycolytic enzymes with energy demand and general functional development in human fetal heart in ontogeny are evaluated.
Volume 10 Issue 3 September 1986 pp 319-322
A comparative study on the activity profile of catalase and superoxide dismutase, the two scavenging enzymes, as well as the developmental profile of lipid peroxidation in the human fetal brain, liver and kidney have been done for gestation periods ranging from 12 weeks to 28 weeks and beyond. The activity of the scavenging enzymes increase gradually inall the tissues with the advancement of pregnancy. Brain is an exception in case of catalase where the activity remains more or less same throughout the developmental period except in the case of fetuses, 28 weeks and above where significant decrease in the catalase activity is observed. A high level of lipid peroxidation is observed during early stages of development which declines thereafter.
Volume 11 Issue 1-4 March 1987 pp 275-285
An amylase was purified from the culture filtrate of
Volume 12 Issue 2 June 1987 pp 99-102
Measurements have been made of the enzymes associated with γ-glutamyl cycle
Volume 13 Issue 2 June 1988 pp 117-121
The ontogeny of glycogen synthetase, glycogen Phosphorylase and α-D-glucosidase, enzymes which are associated with glycogen metabolism and glycogen level has been studied in human fetal testes of gestational age ranging from 14–32 weeks. Glycogen synthetase activity reaches the peak value at 17–20 weeks of gestation, thereafter it decreases. α-D-Glucosidase activity increases with the advancement of pregnancy up to 28 weeks of gestation decreasing thereafter very rapidly. Phosphorylase activity remains more or less constant throughout gestation. The maximum increase in glycogen content at early stages of gestation (17–20 weeks) and gradual reduction with the advancement of pregnancy are correlated with histochemical observation by the periodic acid-Schiff technique.
Volume 15 Issue 3 September 1990 pp 235-238
Liposome-coupled lepromin was found to elicit a 3-week skin reaction in leprosy patients similar to that elicited by whole
Volume 19 Issue 5 December 1994 pp 557-564
Mismatches in DNA occur either due to replication error or during recombination between homologous but non-identical DNA sequences or due to chemical modification of bases. The mismatch in DNA, if not repaired, result in high spontaneous mutation frequency. The repair has to be in the newly synthesized strand of the DNA molecule, otherwise the error will be fixed permanently. Three distinct mechanisms have been proposed for the repair of mismatches in DNA in prokaryotic cells and gene functions involved in these repair processes have been identified. The methyl-directed DNA mismatch repair has been examined in
Volume 22 Issue 1 March 1997 pp 111-116
Volume 27 Issue 7 December 2002 pp 687-693 Articles
Formoguanamine (2,4-diamino-s-triazine) was known to be an effective chemical agent in inducing blindness in poultry chicks, but not in adult birds. The present study was undertaken to demonstrate the influences, if any, of this chemical on the visual performance and retinal histology in an adult sub-tropical wild bird, the roseringed parakeet (
Volume 28 Issue 5 September 2003 pp 533-534 Clipboard
Volume 30 Issue 3 June 2005 pp 377-390
Present work illustrates a scheme of quantitative description of the shape of the skull outlines of temnospondyl amphibians using bilaterally symmetric closed Fourier curves. Some special points have been identified on the Fourier fits of the skull outlines, which are the local maxima, or minima of the distances from the centroid of the points at the skull outline. These points denotes break in curvature of the outline and their positions can be compared to differentiate the skull shapes. The ratios of arc-lengths of the posterior and lateral outline of 58 temnospondyl skulls have been plotted to generate a triaguarity series of the skulls. This series grades different families, some of their genera and species as well as some individuals according to their posterior and lateral skull length ratios. This model while comparing different taxa, takes into account the entire arc-length of the outline of the temnospondyl skulls, and does not depend on few geometric or biological points used by earlier workers for comparing skull shapes.
Volume 32 Issue 7 December 2007 pp 1307-1316 Articles
Docking mode of delvardine and its analogues into the p66 domain of HIV-1 reverse transcriptase: screening using molecular mechanics-generalized born/surface area and absorption, distribution, metabolism and excretion properties
Delvardine and its structural derivatives are important non-nucleoside HIV-1 reverse transcriptase inhibitors (NNRTIs). In this work, 15 delvardine analogues were studied. A free energy-of-binding (FEB) expression was developed in the form of an optimized linear combination of van der Waal (vdW), electrostatic, solvation and solvent-accessible surface area (SASA) energy terms. The solvation energy terms estimated by generalized born/surface area (GB/SA) play an important role in predicting the binding affinity of delvardine analogues. Out of 15 derivatives, substitution of CH3 with H at the Y and R positions, as well as substitution of SO2CH3 with only CH2 at the Z position in S2, S8 and S12 analogues, were found to be the most potent (glide score = –7.60, –8.06 and –7.44; pIC50 = 7.28, 7.37 and 7.64) in comparison with the template delvardine (which is used currently as the drug candidate). All the three analogues also passed the absorption, distribution, metabolism and excretion (ADME) screening and Lipinski’s rule of 5, and have the potential to be used for second-generation drug development. The work demonstrates that dock molecular mechanics-generalized born/surface area (MM–GB/SA–ADME) is a promising approach to predict the binding activity of ligands to the receptor and further screen for a successful candidate drug in a computer-aided rational drug design.
Volume 33 Issue 2 June 2008 pp 303-306 Mini-review
Volume 35 Issue 2 June 2010 pp 281-294 Articles
An insight into the sequential, structural and phylogenetic properties of banana 1-aminocyclopropane-1-carboxylate synthase 1 and study of its interaction with pyridoxal-5'-phosphate and aminoethoxyvinylglycine
In banana, ethylene production for ripening is accompanied by a dramatic increase in 1-aminocyclopropane-1-carboxylate (ACC) content, transcript level of
Volume 36 Issue 4 September 2011 pp 555-557
Volume 38 Issue 2 June 2013 pp 409-412 Mini-reviews
The adaptability of bacteria to extreme cold environments has been demonstrated from time to time by various investigators. Metabolic activity of bacteria at subzero temperatures is also evidenced. Recent studies indicate that bacteria continue both catabolic and anabolic activities at subzero temperatures. Implications of these findings are discussed.
Volume 40 Issue 4 October 2015 pp 721-730 Articles
Reduction of dimensionality has emerged as a routine process in modelling complex biological systems. A large number of feature selection techniques have been reported in the literature to improve model performance in terms of accuracy and speed. In the present article an unsupervised feature selection technique is proposed, using maximum information compression index as the dissimilarity measure and the well-known density-based cluster identification technique DBSCAN for identifying the largest natural group of dissimilar features. The algorithm is fast and less sensitive to the user-supplied parameters. Moreover, the method automatically determines the required number of features and identifies them. We used the proposed method for reducing dimensionality of a number of benchmark data sets of varying sizes. Its performance was also extensively compared with some other well-known feature selection methods.
Volume 43 Issue 2 June 2018 pp 417-419 Mini-Review
The genome of higher eukaryotes is non-randomly organized in the interphase nucleus. However, notwithstanding theabsence of membrane bound sub-compartments, the nucleus coordinates a number of functions largely by organizingchromatin in a non-random but dynamic manner. The plasticity of chromatin structure and function relies on epigeneticmodifications as well as its association with nuclear landmarks such as the nuclear envelope, nuclear lamina, nuclear porecomplex and nuclear bodies such as the nucleolus among others. In the absence of membrane-bound compartments, cellsand the nucleus, in particular, employ phase-separation, which unmixes phases that constrain biochemical reactions incomplex non-membranous sub-compartments such as the nucleolus or even the heterochromatin. This review attempts toprovide a glimpse into the microcosm of phase-separated nuclear sub-compartments, that regulate nuclear structure–function relationships.
Volume 43 Issue 5 December 2018 pp 1001-1013 Article
The ribosome-binding GTPase HflX is required for manganese homeostasis in E. coli. While under normal conditionsDhflX cells behave like wild type E. coli with respect to growth pattern and morphology, deletion of hflX makes E. coli cellsextremely sensitive to manganese, characterized by arrested cell growth and filamentation. Here we demonstrate that uponcomplementation by hflX, manganese stress is relieved. In phenotypic studies done in a manganese-rich environment, DhflXcells were highly sensitive to antibiotics that bind the penicillin binding protein 3 (PBP3), suggesting that the manganesestress led to impaired peptidoglycan biosynthesis. An irregular distribution of dark bands of constriction along filaments,delocalization of the dark bands from midcell towards poles and subpoles, lack of septum formation and arrested celldivision were observed in DhflX cells under manganese stress. However, chromosome replication and segregation ofnucleoids were unaffected under these conditions, as observed from confocal microscopy imaging and FACS studies. Weconclude that absence of HflX leads to manganese accumulation in E. coli cells, affecting cell septum formation, probablyby modulating the activity of the cell division protein PBP3 (FtsI), a major component of the divisome apparatus. Wepropose that HflX acts as a gatekeeper, regulating the influx of manganese into the cell.
Volume 44 Issue 1 March 2019 Article ID 0022 Review
Cancer is a physiological condition that has both the endogenous and exogenous influences on its progression. It originatesfrom unusual cell growth, where the cells undergo massive genetic alterations, bypass the signaling machinery andcompromise its genetic cohesion. Literature has well narrated the DNA damage studies including driver mutations thatinterfere with the treatment strategies. However, with evolving medical excellence, recent day studies are trying to unveilthe contribution of RNAs in the progression of tumor malignancies. A number of non-coding RNAs have been identified asan active component in cancer genomics. This article aims to review the role of long non-coding RNAs in the spectra ofcancers and its prognostic value as the biomarkers in molecular targeting with clinical utility and therapeutic beneficence.
Volume 45 All articles Published: 20 January 2020 Article ID 0018 Review
The mammalian genome is complex and presents a dynamic structural organization that reflects function.Organization of the genome inside the mammalian nucleus impacts all nuclear processes including but notlimited to transcription, replication and repair, and in many biological contexts such as early development,differentiation and physiological adaptations. However, there is limited understating of how 3D organization ofthe mammalian genome regulates different nuclear processes. Recent advances in microscopy and a myriad ofgenomics methods—propelled by next-generation sequencing—have advanced our knowledge of genomeorganization to a great extent. In this review, we discuss nuclear compartments in general and recent advancesin the understanding of how mammalian genome is organized in these compartments with an emphasis ondynamics at the nuclear periphery.
Volume 45 All articles Published: 18 January 2020 Article ID 0002 Article
Accumulating evidences indicate the involvement of epigenetic deregulations in cancer. While some epigeneticregulators with aberrant functions in cancer are targeted for improving therapeutic outcome in patients,reinstating the functions of tumor-suppressor-like epigenetic regulators might further potentiate anti-cancertherapies. Epigenetic reader zinc-finger MYND-type-containing 8 (ZMYND8) has been found to be endowedwith multiple anti-cancer functions like inhibition of tumor cell migration and proliferation. Here, we reportanother novel tumor suppressor role of ZMYND8 as an inducer of differentiation in breast cancer cells, byupregulating differentiation genes. Interestingly, we also demonstrated that ZMYND8 mediates all its antitumorroles through a common dual-histone mark binding to H4K16Ac and H3K36Me2. We validated thesefindings by both biochemical and biophysical analyses. Furthermore, we also confirmed the differentiationinducingpotential of ZMYND8 in vivo, using 4T1 murine breast cancer model in Balb/c mice. Differentiationtherapy holds great promise in cancer therapy, since it is non-toxic and makes the cancer cells therapysensitive.In this scenario, we propose epigenetic reader ZMYND8 as a potential therapeutic candidate fordifferentiation therapy in breast cancer.
Volume 45, 2020
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